Comparison of <i>in vitro</i> Maturation Media on Cattle Oocytes after <i>in vitro</i> Embryo Production

The study was conducted to compare differences in vitro maturation media and their effect on subsequent embryo production. procedures were approved by the agricultural research council animal ethics committee (Ref no: APAEC 2020/05) Tshwane university of technology AREC 2021/08/004). Follicular fluid containing a suspension oocytes retrieved from ovaries through aspiration slicing techniques. graded randomly matured into four (TCM199, Vitromat Protect, BO IVM®) groups for 22 h, evaluated polar body extrusion, fertilized respective fertilization media, pronucleus status. Presumptive zygotes further cultured incubated either 18 or 96 cleavage rates. data subjected an appropriate analysis variance. Student's Least Significant Differences (t-LSD) calculated at 5% significance level means significant treatment effects. BO-IVM® (57.4%), TCM 199 (55.6%), VitroMat Protect® (54.0%) recorded higher results than EGF (26.9%) formation (P<0.05). Vitrofert® medium percentage (43.3%) total rate when compared other VitroCleave Plus® as In conclusion, 199, BO-IVM®, rendered oocyte extrusion rates medium. media. Higher development (4-8 cells cleavage) observed Overall, introduced (VitroMat Protect®) its suite successfully adapted laboratory environment South Africa (SA) can therefore be adopted optimizing Embryo Production (IVEP) cattle oocytes.